Chemically Competent Cells Overview
The Mix & Go Competent Cells from Zymo Research offer the simplest and most efficient means of E. coli transformation. Zymo Research’s scientists have developed a unique method that completely eliminates the need for heat shock, lengthy incubations, and outgrowth steps. Simply mix DNA with the cells then spread directly to a plate… Mix & Go! The competent cells are highly efficient and can be used for cloning, sub-cloning, library construction, etc. These can be purchased as individual 100 µl aliquots or as 50 µl aliquots in a 96-well format.
XJ Autolysis™ E. coli offer a new alternative for recombinant protein expression and lysis. Autolysis strains are efficiently lysed following treatment with arabinose and the induced expression of bacteriophage λ endolysin. Coupled with a single freeze-thaw step, the cells greatly simplify protein expression and purification. They are also available with a DE3 lysogen encoding the T7 polymerase for expressing recombinant proteins driven by the T7 promoter, and all Autolysis strains are suitable for nucleic acid purification.